Mice were immunized with LP1569 (50 μg) + cGMP (10 μg) only; vaccine only, consisting of LP1569 (LP) + cGMP + ClfA (5 μg); vaccine + anti–IL-10 (150 μg); or vaccine + isotype control (150 μg), following the same regimen of injections and subsequent s.c. infection on day 42 with LAC (2 × 107 CFU). This evidence concerns the gene LCT and infection.