LAG3 and neoplasm: In addition, recombinant mouse FGL1 protein had no direct effect on the proliferation (Supplemental Figure 5A) or migration (Supplemental Figure 5B) of MC38, B16-F10, and Hepa1-6 tumor cells in vitro, suggesting that the FGL1/LAG-3 interaction did not play a direct role in tumor metastasis or growth in our settings and further indicating the critical roles of CD8+ T and NK cells in the antitumor efficacy of FGL1 blockade.