It has been reported that solitary CD86 exhibits the capability to directly activate NK cells, independent of other co‐stimulatory molecules.[42] Concurrently, as a co‐stimulatory signal, CD86 can strengthen the anti‐tumor immune response by enhancing the activity of CD8+T‐cells.[43, 44] Notably, recent findings indicate that NK cells, through the NK‐cDCs‐T‐cell axis, play a pivotal role in recruiting T‐cells, thereby amplifying the cytotoxic impact on tumors.[45, 46] In light of these discoveries, we designed a recombinant plasmid, connecting the CD86 and EGR3 genes through the P2A peptide. Here, CD8A is linked to neoplasm.