AIF1 and neoplasm: In CDNP-R848 treated mice, TAMs were more abundant in tumor areas with low stiffness compared to areas of intermediate and high stiffness (mean percentage of iba1-positive tumor volume in areas with low, intermediate and high stiffness: 8.78, 1.78 and 0.15%; p < 0.01 and p < 0.001 when comparing subregions of low to subregions of intermediate and high stiffness, respectively; Fig. 5C).