In contrast, the lentiviral scramble control shRNA plus the CRISPR/Cas9 control construct (“shC+Cas9-C”) treatment failed to activate caspase-Cytochrome C pathway (Fig. 5A–D), induce mitochondrial depolarization (Fig. 5E), or trigger apoptosis (Fig. 5F, G), and subsequent cell death (Fig. 5H) in P1 glioma cells. Here, CYCS is linked to glioma.