ORC6 and central nervous system cancer: In order to study the potential role of ORC6 in glioma cell growth in vivo, the P1 glioma cells expressing the CRISPR/Cas9-ORC6-KO construct (“koORC6”) or the Cas9-C control (“Cas9-C”) were injected intracranially into the brains of nude mice (according to the previously-described protocols [28, 31, 32]).