Using a series of biochemical approaches, RNAi, and RNA-seq, the data presented here support that FMRP does stall ribosomes and stimulates NGD in neuroblastoma cells on a small number of transcripts, revealing a minor role of FMRP that would be misregulated in FXS when FMRP is lost. This evidence concerns the gene FMR1 and fragile X syndrome.