Here, compared to that in the matched control Hepa1-6 tumors, the infiltration of TAMs was significantly reduced in MELK-inhibited Hepa1-6 tumors; in these tumors, the proportion of protumor- and immunosuppressive-related M2 phenotypes was especially reduced, while the proportion of M1 phenotype macrophages, which play a role in tumor suppression, was substantially elevated. The gene discussed is MELK; the disease is neoplasm.