To investigate the impact of the CC domain on the influence of RNF214 on the growth of HCC cells, Venus, Venus-RNF214, and Venus-∆CC were reintroduced into RNF214-deficient HCC cells (SK-HEP-1 RNF214-sg2 and MHCC97H RNF214-sg2) via stable expression (Fig. 6a, b). The gene discussed is RNF214; the disease is hepatocellular carcinoma.