To further confirm whether the CC domain also mediates the phase separation of RNF214 formation in HCC cells, MHCC97H cells were transfected with Venus, Venus-RNF214, Venus-ΔCC, and Venus-CC expressing vectors and treated with 0.8 M sorbitol or 250 mM NaCl for 10 min. This evidence concerns the gene RNF214 and hepatocellular carcinoma.