LRMDA and melanoma: Most strikingly, cluster 12_MBMMac, in which the top differentially expressed gene was LRMDA, a known melanocyte differentiation factor, was significantly highly enriched (moderated two-sided ANOVA via propeller87 with FDR correction, q = 0.00000000102) in melanoma brain metastases compared to both primary melanomas and primary glioblastomas (Fig. 3c, d), suggestive of an interaction between this tumour genotype and the surrounding brain tissue.