Most strikingly, cluster 12_MBMMac, in which the top differentially expressed gene was LRMDA, a known melanocyte differentiation factor, was significantly highly enriched (moderated two-sided ANOVA via propeller87 with FDR correction, q = 0.00000000102) in melanoma brain metastases compared to both primary melanomas and primary glioblastomas (Fig. 3c, d), suggestive of an interaction between this tumour genotype and the surrounding brain tissue. Here, LRMDA is linked to glioblastoma.