To determine whether specific immune cell types were enriched in the ING4-deficient tumor microenvironment (TME), we first employed immunohistochemical (IHC) staining of breast tumor tissue microarrays (TMAs) for selective immune markers: CD68 (macrophages), CD4 (helper T cells), CD8 (cytotoxic T cells) and PD-1 (an immune checkpoint molecule on lymphocytes). This evidence concerns the gene CD8A and neoplasm.