Armstrong et al. (2016) modeled ALS in zebrafish by creating site-specific single nucleotide mutations for the first time in two ALS-related genes tardbp and fus using CRISPR-Cas9 mediated homology-directed repair by co-injection of single-stranded oligodeoxynucleotide donor templates (ssODN) with gRNA and Cas9 mRNA. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.