There are several other BRAF hot-spot codons located in exon 11 (G464, G466, G469) or exon 15 (D594, G596, L597, K601), which are relevant to LC pathogenesis, however, they cannot be efficiently targeted by currently available therapies, and, therefore, are not mandatory for LC molecular testing [63, 64]. Here, BRAF is linked to laryngotracheoesophageal cleft.