In the in vitro cytotoxicity study, the anti-GPC1 mAb cytotoxicity was found in an ascending order with H460 cell monocultures (mean IC50 = 646 μg/mL) < A549 cell monocultures (mean IC50 = 169 μg/mL) < LL97A fibroblast monocultures (mean IC50 = 60 μg/mL), indicating that lung fibroblasts are more sensitive to the cytotoxic effect of anti-GPC1 mAb than NSCLC cells (Figure 1). The gene discussed is GPC1; the disease is non-small cell lung carcinoma.