Next-generation sequencing technology was used to analyze the genes of 404 cases of focal epilepsy, with two pathogenic mutations of the NPRL2 gene detected in two patients with familial SHE (p. Leu105Pro and p. Arg34*) and one pathogenic mutation of the NPRL3 gene (p.Ser279Phefs*52) identified in a familial case of SHE. This evidence concerns the gene NPRL3 and sleep-related hypermotor epilepsy.