The behavioral tests were performed two weeks after virus injection, and showed reductions in anxiety‐like behaviors in LV‐shRNA‐nNOS‐treated rats compared with that in LV‐shRNA‐eGFP group, including increased time, entries and distance in the center zone of OFT (Figure 5G,H) and in the open arm of EPM (Figure 5I,J). This evidence concerns the gene NOS1 and Anxiety.