It was found that the surface expression of GABAAR γ2 was increased in LV‐shRNA‐nNOS compared with that in control LV‐shRNA‐eGFP group, without changes in total level of GABAAR γ2 (Figure 5K–M), suggesting that nNOS knockdown‐mediated reduction of SNO‐gephyrin ameliorates anxiety‐like behaviors by increasing surface clustering of GABAAR γ2. This evidence concerns the gene GPHN and Anxiety.