RNA-sequencing studies (GSE214471) indicated that many epithelial biomarkers (EPCAM, CDH1, C1ORF116, S100A14, ZNF165, and KDF1) were greatly upregulated (p < 0.001), suggesting that ZEB1 expression has been successfully knocked down in GC cell lines (Fig. 1b). This evidence concerns the gene C1orf116 and gastric cancer.