However, the low tissue specificity of these proteins, particularly to AT, raises concerns about their reliability as AT EV markers, as TGFB1 is also expressed by immune cells and found in monocyte-derived EVs [47], and it has been associated with other inflammatory conditions and diseases, such as adenocarcinoma as well as pulmonary hypertension in HIV [48, 49]. This evidence concerns the gene TGFB1 and pulmonary arterial hypertension.