This study revealed that BP-PEI nano-sheets could effectively transport RNAs into PC3 cells and acted as “endogenous sponges” to competitively bind miRNAs which could interact with PTEN mRNA and induce its degradation to elevate PTEN expression, and it could dramatically inhibit cell proliferation and enhance cell apoptosis, autophagy and cell cycle arrest to exert an obviously anti-tumor effect on PCa. Here, PTEN is linked to neoplasm.