Overall, all E. faecalis carried at least 14 virulence genes including multiple adhesins and biofilm-associated genes like [ace (collagen adhesin), cad, camE, cCF10, cOB1 (sex pheromone-associated genes), ebpA/B/C (endocarditis and biofilm-associated pili), efaAfc (cell wall adhesion expressed in serum), ElrA (leucine-rich protein A associated with macrophage persistence), hylA, gelE, SrtA (gelatinase with protease activity), tpx(thiol peroxidase for oxidative stress resistance), fsrB (gelE expression)]. The gene discussed is TPO; the disease is endocarditis.