To functionally validate this, we treated a panel of TNBC cell lines - MDA-MB-231, Hs578T and BT549, HER2 cell lines - SKBR3 and MDA-MB-453, and ER-positive BC cell lines - MCF7, T47D and CAMA1, with exogenous TWEAK ligand. This evidence concerns the gene TNFSF12 and breast cancer.