As LFMRS was closely related to the immunosuppressive state of AML (Fig. 2O), we further explore whether LGALS1 regulates the immune effect of immune cells (macrophage, CD8+ T cells, and NK cells) in vivo by using the MLL-AF9 retroviral transduction system (Fig. 6F). The gene discussed is CD8A; the disease is acute myeloid leukemia.