Such loss of synapses was neither associated with changes in the expression of microglial genes linking neuronal A2AR upregulation to tau-induced synapse loss such as C1qa, Pycard and Csf1r (Supplementary Fig. 1, 2A) nor with the engulfment of synapses by microglial cells (Supplementary Fig. 1, 2B), suggesting that the loss of hippocampal synapses and associated impaired memory found in APP/PS1 A2A mice is not subsequent to a microglial-based pruning as we previously observed in a tauopathy context. Here, PYCARD is linked to tauopathy.