Through fluorescence-activated cell sorting (FACS), we identified a distinct zebrafish cell population with ALDHHigh activity that was also low for mitfa:GFP (Figure 7B), indicating that both zebrafish and human melanoma ALDHHigh cells express low levels of MITF. Next, we sorted zebrafish melanoma cells with the highest and lowest ALDH activity and performed RT-qPCR (Figures 7C and 7D). The gene discussed is LDHA; the disease is melanoma.