Knockdown of GBE1 significantly reduced cell growth as a smaller tumor size was observed in GBE1 knockdown group (Fig. 3I, J, Fig. S2B), and the body weight change of nude mice was shown in Fig. 3K. To further confirm the role of GBE1 in PC cell tumorigenesis, we orthotopically injected PC cells into the pancreas of nude mice. This evidence concerns the gene GBE1 and pachyonychia congenita.