To validate the results obtained from the analysis, the accuracy and reliability of our analysis were further verified by establishing a mouse model with diabetic cardiomyopathy and detecting hub genes (Pdk4, Lipe, Igf1r, Serpine1, and Bcl2l1) through RT-qPCR to validate the results obtained from the analysis. This evidence concerns the gene PDK4 and diabetic cardiomyopathy.