ANXA5 and cervical carcinoma: Additionally, we conducted annexin-PI staining to evaluate whether bergenin is inducing apoptosis in cervical cancer cells. In Fig. 1D, upon treatment in siHa cells with 100 μM and 200 μM of bergenin for 48 h, we observed indications of early apoptosis, late apoptosis, and cell necrosis using annexin-V-PE/7-AAD double staining.