However, there were no significant differences between VEN-sensitive and resistant cells analyzing the overall number of mitochondria per cell, mitochondrial mass (MitoTracker incorporation), polarization of the mitochondrial outer membrane (TMRM positivity), or expression of the mitochondrial transcription factor A (TFAM), which regulates transcription, replication, and packaging of mtDNA [29] (Supplementary Fig. 12A), arguing against an overall increased mitochondrial biogenesis in VEN-resistant ALL. The gene discussed is TFAM; the disease is acute lymphoblastic leukemia.