ALK and non-small cell lung carcinoma: We therefore cloned the EML4/ALK translocation (EML4/ALK variant) as well as the p.Arg1181His missense mutation (EML4/ALKR1181H variant) into an NSCLC cell line (BaF3) via the retroviral vector PIG (plasmid-IRES-EGFP-Puromycin).