FOS and Anorexia: BTD administration increased c-Fos immunoreactivity in ARH Pomc neurons (Figure 4D) and chemogenetic inactivation (designer receptors exclusively activated by designer drugs [DREADD]; STAR Methods) of hM4Di-expressing Pomc neurons with clozapine N-oxide (CNO) blocked BTD-induced anorexia (Figures 4E, 4F, and S4E–S4G).