PDX1 and familial pancreatic carcinoma: Here, we adapt ISDoT (in situ decellularization of tissues) technology (34, 35) to enrich for matrisomal proteins, coupled with label-free data-independent acquisition liquid chromatography–tandem MS (DIA LC-MS/MS) to dissect the fibrotic signatures of pancreatic tumors from two commonly used genetically engineered mouse models (GEMMs) of pancreatic cancer: the highly metastatic KPC (Pdx1-Cre, LSL-KrasG12D/+, LSL-Trp53R172H/+) and poorly metastatic KPflC (Pdx1-Cre, LSL-KrasG12D/+, Trp53fl/+) mouse models (36, 37).