TBCE and metabolic dysfunction-associated steatotic liver disease: Kostrzewski et al. (2020) developed an in vitro human liver model using a three-dimensional MPS with a coculture of microtissues containing primary human hepatocytes, KCs, and stellate cells to represent NASH.120 NASH is a severe form of NAFLD caused by a buildup of fat in the liver that can eventually lead to cirrhosis and liver cancer.