Another study evaluating NASH, recreated the early stages of this disease using a MPS design that integrated hepatic sinusoidal flow, transport, and lipotoxic stress risk factors (glucose, insulin, free fatty acids) with primary human hepatocytes, HSCs, and KCs.125 They found fibro-genic activation markers such as TGF-β, ECM gene expression, and HSC activation, increased with lipotoxic conditions. The gene discussed is TBCE; the disease is metabolic dysfunction-associated steatohepatitis.