Additionally, pcDNA3.1-MIR497HG downregulated the miR-16-5p level in BT549 and Hs 578T cells in Fig. 3E. Spearman analysis assessed that the expression of MIR497HG was negatively correlated with the expression of miR-16-5p in breast cancer tissues (r = -0.7037, P < 0.0001), as demonstrated in Fig. 3F. Taken together, MIR497HG reversely regulates the downstream target miR-16-5p to affect the progress of breast cancer. Here, MIR497HG is linked to breast cancer.