MLANA and cancer: We performed antigen discovery screens against the human peptidome combining SrtA-Jurkat cells expressing the cancer/testis antigen-reactive IG4 or DMF5 TCRs (A2+/melanocyte antigen (MLANA)/EAAGIGILTV), with three additional HLA-A2-restricted TCR clonotypes that do not exhibit reactivity to self-antigens contained in the human peptidome library (Fig. 4c).