CD27 and neoplasm: To define the B cell phenotypes that expressed elevated CEACAM1 levels in relation to tumor-associated disease progression, we first used manual gating strategies to parse the viSNE coordinates into naive (NB; CD19+IgD+CD27−), memory (MB; CD19+CD27+), plasmablastic (PBL; CD27+CD38+CD20−), activated (Act; IgD+CD38+) and double-negative (DN; IgD−CD27−) subsets and identified these populations within the viSNE coordinates (Supplementary Fig. 4a).