Metacluster C58748 represented CD4+CD45RO+ T cells that expressed CCR6, CCR7, CXCR3 and CXCR5 suggesting TFH cells in melanoma (Fig. 6n) which exhibit elevated CEACAM1 levels in the context of treatment-resistant compared to treatment-naive disease (Fig. 6o). Here, CXCR3 is linked to melanoma.