This showed that CEACAM1 in the tumor samples was associated with metaclusters which were annotated as memory (M2, M5), naive-memory (M6, M8, M10; CD19+IgD+CD27+) and DN (M7) B cells in the treatment-naive and -resistant samples based upon the channel-colored marker levels in the MST (Fig. 3h, Supplementary Fig. 4c), the scaled marker expression in a heatmap (Fig. 3i) and an overlay of the MST and manually gated output (Supplementary Fig. 4d). This evidence concerns the gene CD27 and neoplasm.