To verify that this nested patchwork of MSH6 protein labeling faithfully reflected the MSH6 genotype, we carried out detailed laser capture microdissection (LCM) followed by Sanger sequencing of the MSH6-deficient lineage, the MSH6-proficient nested subclone and background MSH6-proficient tumor cells from each of three tumors. This evidence concerns the gene MSH6 and neoplasm.