ITGB1, a major integrin subfamily member, is a cell surface receptor involved in various biological processes.46 It is aberrantly expressed in many tumors, including HCC, where it promotes tumor progression by regulating the cell cycle.22 Our study showed that inhibition of ITGB1 also antagonized Sema3C-mediated stemness characteristics, and signatures constructed with ITGB1, NRP1, and Sema3C expression also suggested that high signatures had the worst prognosis in HCC patients. Here, SEMA3C is linked to hepatocellular carcinoma.