IHC analysis of proliferative marker Ki67, Collagen I, and EPCAM in the excised livers from the four treatment groups revealed a marked decrease in the rAAV8-shSema3C/sorafenib combination group, suggesting knocked down of Sema3C did effectively impair proliferative capacity, stromal deposition, and stemness niche of the tumor (Fig. 8h). This evidence concerns the gene MKI67 and neoplasm.