Western blot results showed that the levels of cleaved PARP, cleaved caspase3 and γ-H2AX were increased and Bcl-2 was decreased with increasing concentrations of RSL3 in both MDS-L and SKM-1 cells (Fig. 1F), further confirming RSL3 induces apoptosis in MDS cells. This evidence concerns the gene H2AX and myelodysplastic syndrome.