This study demonstrated that paclitaxel and doxorubicin, an anthracycline, induced pyroptosis in breast cancer cells with high GSDME expression, accompanied by GSDME cleavage, activation of caspase-3 and upstream caspase-9 proteins in the mitochondrial-mediated death pathway, LDH release and increased Annexin V+/PI+ cells. The gene discussed is CASP9; the disease is breast cancer.