To assess the ability of GSDME-mediated pyroptosis to promote anti-tumor immunity of neoadjuvant chemotherapy in breast cancer, paclitaxel-treated MDA-MB-231 cells with high GSDME expression were cocultured with mature DCs and CD3+ T cells for 24 h, and then IFN-γand IL-2 secretion levels in the supernatant were detected by ELISA, respectively. This evidence concerns the gene GSDME and breast cancer.