Interestingly, a slight increase in the expression of the DNA damage marker p21 was observed upon SAVI THP1 CM treatment (Fig. 6 H) with concomitant increase in phosphorylation of H2AX (Fig. 6, I and J), increased cleaved Caspase-3 signal (cC3) (Fig. 6, I and K), and reduced cell migration (Fig. S4, B and C), overall highlighting cytokine-mediated endothelial dysfunction, cell damage, and apoptosis. Here, CASP3 is linked to endothelial dysfunction.