To functionally assess whether tumor cell‐expressed SRC‐1 could inhibit the antitumor activity of CD8+ T cells, a co‐culture with effector CD8+ T cells and Ctrl or SRC‐1‐deficient CRC cells in vitro was performed, and then the tumor cell death and antitumor activity of CD8+ T cells were assessed by Annexin V/PI and GZMB/TNFα staining, respectively. The gene discussed is ANXA5; the disease is colorectal carcinoma.