Several studies have reported that deficiency of PC related to a choline-deficient diet or knockout of CCTα or PEMT specifically in the liver leads to hepatic TG accumulation full of large LDs, which is a hallmark signature of NAFLD (Jacobs et al., 2004; Li et al., 2006; Guo et al., 2008; Krahmer et al., 2011). This evidence concerns the gene PCYT1A and metabolic dysfunction-associated steatotic liver disease.