Synergistic immunotherapy with NGC-Gel and TIM-3 blockers induced a significant reduction in regulatory T cells in tumor tissues, overexpression of IFN-γ and lL-12p70, increased proportions of IFN-γ+CD8+T cells and 41BB+CD8+T cells, and a reduction in Foxp3+CD25+T cells. The gene discussed is IFNG; the disease is neoplasm.