Results suggested that Gli1 PEI-SNAs were successfully picked up by U-87 MG cells via scavenger receptors, silenced the expression of Hedgehog signaling pathway genes like Gli1 and Smo (∼ 30%), subsequently leading to a ∼ 30% reduction in expression of downstream transcriptional target genes of Gli1 (e.g., CyclinD1, c-Myc, Bcl2, and ABCG2), and eventually, prevented the progression of chemotherapy-resistant GBM by reducing the proliferation of GBM cells (30%), as well as their metabolic activity (∼ 60%) and self-renewal capability (30–40%) (Table 2) [146]. This evidence concerns the gene ABCG2 and glioblastoma.