Genetically-proxied GK activation was not causally associated with risks of MASLD (OR 0.76, 95% CI 0.42–1.39, P = 0.374), PAD (OR 0.86, 95% CI 0.42–1.76, P = 0.681) or stroke (OR 1.00, 95% CI 0.70–1.43, P = 0.995). This evidence concerns the gene GK and stroke disorder.