Accordingly, we imaged live human colon adenocarcinoma HT29 cells with combinations of DiI and either the transferrin receptor (TfR), which continuously recycles to the plasma membrane after endocytosis in CCPs, where it is enriched28, or CD59, a GPI-anchored protein, whose clustering status together with other proteins implicated in T cell signalling is controversial9,23,29,30. Here, CD59 is linked to colon adenocarcinoma.