To investigate whether the Ctcf+/R567W-induced phenotype in mice could replicate clinical neurodevelopmental disorders and autism-like traits, we conducted a series of behavioral experiments, including the open-field test (OFT), novel object recognition (NOR) test, three-chamber test, elevated plus maze (EPM) test, morris water maze test, and rotarod test. This evidence concerns the gene CTCF and autism.