Among the ccRCC cell lines, OSRC2 and 769-P showed the highest and lowest ISG15 expression levels, respectively, as indicated by the results in Fig. 1F, G. Stable ISG15-knockdown cell lines were created using the OSRC2 cell line, while stable ISG15-overexpression cell lines were generated using the 769-P cell line (Fig. 1H–K). This evidence concerns the gene ISG15 and nonpapillary renal cell carcinoma.