Nonetheless, the species clustering heatmap, the number of group or sample OTUs, and the Shannon index also showed significant differences in salivary microbiota between DGF and IGF patients even though at similar levels of Scr, suggesting that DGF-related acute kidney injury, secondary systemic inflammation, etc. significantly altered the distribution of salivary microbiota in patients. This evidence concerns the gene IGF1 and acute kidney injury.