Therefore, mice bearing two subcutaneous tumors (Supplementary Fig. 3F) were treated with IR to only one of the tumors (including concomitant or delayed DLN IR) in order to probe the efficacy of the combined radioimmunotherapy regimen also against the distant, non-irradiated tumor, again using α-CTLA-4 and a single high dose of IR. This evidence concerns the gene CTLA4 and neoplasm.