As evidenced in the “TM + NEO-DLN IR + ICI” treatment group, increased proliferation was present already on day 4 after neoadjuvant DLN IR (which corresponds to day -3 relative to tumor IR) (Fig. 7G), with an almost twofold increase in the proportion of proliferating CD8+ T cells in comparison to non-irradiated lymph nodes (6.74 ± 0.54% and 3.91 ± 0.17%, respectively). Here, CD8A is linked to neoplasm.