Compared to the “Sham IR + ICI+ and “TM + C-DLN IR + ICI” groups, a trend towards a higher expression of PD-1 and TIM-3 could be observed on tumor-infiltrating CD8+ T cells isolated from mice in the “TM IR + ICI” group both on day 2 and day 7 after tumor IR. The gene discussed is CD8A; the disease is neoplasm.