This suggests that monitoring the methylationlevels of these indicators in oral rinses may be an effective wayto predict recurrence and provide guidance for therapy in people withHNSCC.12 ddPCR method was used to analyzethe PAX5 methylation in deep surgical margin samples from 82 individualswith HNSCC that were histologically cancer-free.13,14. This evidence concerns the gene PAX5 and head and neck squamous cell carcinoma.